2020-07-02
Under the action of an electric field, charged particles move toward an electrode opposite to their electrical properties, called electrophoresis (EP). The technique of using charged particles to move at different speeds in an electric field to achieve separation is called electrophoresis.
In 1936, Swedish scholar A.W.K. Tselius designed and manufactured a mobile interface electrophoresis apparatus, which separated three kinds of globulin of horse serum albumin and created electrophoresis technology.
Application field
Electrophoresis has been widely used in various fields such as analytical chemistry, biochemistry, clinical chemistry, toxicology, pharmacology, immunology, microbiology, and food chemistry. In a DC electric field, the phenomenon of charged particles moving toward electrodes with opposite signs is called electropho-resis. In 1807, Ferdinand Frederic Reuss (Moscow University, Russia) first discovered the phenomenon of electrophoresis, but it was not until 1937 that Tiselius of Sweden established boundary electrophoresis to separate proteins. , Electrophoresis technology began to apply. In the 1960s and 1970s, when media such as filter paper and polyacrylamide gel were introduced into electrophoresis, electrophoresis technology developed rapidly. The colorful electrophoresis forms make it widely used. In addition to the separation and analysis of small molecular substances, electrophoresis technology is mainly used for the study of proteins, nucleic acids, enzymes, viruses and cells. Because some electrophoresis methods are simple, easy to operate, and have high resolution and selectivity, they have become a commonly used technique in medical testing.
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